Fig. 1: Endo180 is associated with fibroblast activation.
From: Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasis
a Immunohistochemical staining of Endo180 (mAb 39.10) of a tumour microarray of invasive human breast cancers26. Illustrative images, scale bar, 0.1 mm. b Endo180 (Mrc2) expression in endothelial cells, fibroblasts, immune cells, macrophages and tumour cells isolated from mouse D2A1-m2 tumours (data from three individual mice; one-way ANOVA with Tukey’s multiple comparison test). c Boxplots of Endo180 (MRC2), ACTA2 and FAP expression in microdissected breast cancer stroma (n = 53 samples) and adjacent normal tissue stroma (n = 6 samples). Box plots shows median and 25th–75th quartiles, whiskers show minimum and maximum. (GSE90134, two-sided Mann–Whitney U-test). d Relative Endo180 (Mrc2) expression in cultured normal mouse mammary gland fibroblasts and two independent CAF cultures isolated from 4T1 tumours (n = 2). e Left panel, relative Endo180 (MRC2) expression in human MRC5 fibroblasts cultured alone or co-cultured with D2A1 or MDA-MB-453 tumour cells. Middle and right panels, Endo180 (MRC2) expression in Wi38 and HFF1 human fibroblasts, respectively, cultured alone or co-cultured with MDA-MB-231 tumour cells (GSE4167872; Wi38 panel and HFFF1 alone, n = 4; HFFF1 co-culture, n = 3; mean values ± SEM, two-sided t-test). f Endo180 (MRC2) expression in fibroblasts isolated from different breast cancer subtypes. Box plots shows median and 25th–75th quartiles, whiskers show minimum and maximum. (GSE3761471; ER+, n = 7; n = 5 HER2+, n = 5; TNBC, n = 6; one-way ANOVA with Tukey’s multiple comparison test, all non-significant (ns) P > 0.05). g Correlation of Endo180 (MRC2) gene expression with a fibroblast TGFβ response signature (F-TBRS)32 in breast cancers from the TCGA (Pearson correlation, r = 0.57, P < 0.0001 n = 522) and the NKI29570 (Pearson correlation, r = 0.56, P < 0.0001, n = 337) datasets. h Endo180 (Mrc2) gene expression in the published single cell sequencing dataset of CAFs isolated from MMTV-PyMT tumours7. Shown is Mrc2 expression log2(RPKM + 1) in individual cells (left panel, tSNE plot) and in the vascular (vCAF, n = 485), matrix (mCAF, n = 144), cycling (cCAF, n = 43) and development (dCAF, n = 44) subpopulations (Violin plots, red bars indicate median expression levels, dotted lines indicate upper and lower quartiles, Kruskal–Wallis test, Dunn’s correction). Source data are provided as a Source Data file.
