Fig. 4: Loss of AT2 MHCII results in greater weight loss and reduced survival after respiratory viral infection.
a–f Comparison of Ab1fl/fl and SPCΔAb1 mice after infection with influenza strain PR8 (IAV PR8) (a, c, e) and Sendai virus strain 52 (SeV 52) (b, d, f). a, b Weight loss with weights displayed relative to day of infection; n = 43 Ab1fl/fl and n = 53 SPCΔAb1 mice pooled from six independent experiments (a), and n = 17 Ab1fl/fl and n = 22 SPCΔAb1 mice pooled from two independent experiments (b). c, d Mortality, with curves representing proportion surviving; n = 22 Ab1fl/fl and n = 21 SPCΔAb1 mice pooled from two independent experiments (c), and n = 17 Ab1fl/fl and n = 22 SPCΔAb1 mice pooled from two independent experiments (d). e, f Lung virus titers on the days post-infection (dpi) indicated; n = 7 mice per strain from one experiment (e), and n = 5 mice per strain for days 4 and 9, n = 11 Ab1fl/fl and n = 12 SPCΔAb1 mice for day 7, pooled from two independent experiments (f). a, b Data are mean plus SEM, analyzed by mixed-effects model with post-hoc multiple comparisons with Sidak’s correction at each dpi. P values displayed represent overall model effects. Full statistical test results are in Supplementary Tables 3–4. c, d Survival curves were compared via Log-rank test (χ2 = 2.327, df = 1 (c), and χ2 = 1.173, df = 1 (d)). e, f Bars are mean plus SEM of log10 transformed values, analyzed by unpaired two-tailed Student’s t-test (t = 1.995, df = 12) (e), and two-way ANOVA (genotype effect: F = 1.559, df.n = 1, df.d = 37, P = 0.2197) with post-hoc multiple comparisons with Sidak’s correction (4 dpi t = 0.6853, df = 37; 7 dpi t = 0.7603, df = 37; 9 dpi t = 0.7614, df = 37). P values displayed represent post-hoc comparisons (f). For all panels, Ab1fl/fl are in gray, SPCΔAb1 in red. Source data are provided as a Source data file.
