Fig. 4: RDM15 physically interacts with NRPE3B, a subunit exclusive to Pol V. | Nature Communications

Fig. 4: RDM15 physically interacts with NRPE3B, a subunit exclusive to Pol V.

From: A histone H3K4me1-specific binding protein is required for siRNA accumulation and DNA methylation at a subset of loci targeted by RNA-directed DNA methylation

Fig. 4

a Detection of proteins associated with RDM15. LC-MS/MS was performed following immunoprecipitation of FLAG-tagged RDM15 and MYC-tagged RDM15. There were two biological replicates for each of the protein purifications. b Tests of RDM15-NRPE3B interaction by split luciferase complementation assay in tobacco leaves. Circles indicate leaf regions that were infiltrated with Agrobacterium strains containing the indicated constructs. (Scale bar = 1 cm) c Analyses of RDM15-NRPE3B protein interaction by bimolecular fluorescent complementation assay in tobacco leaves. Yellow fluorescence indicates positive protein interaction. (Scale bar = 20 µm).

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