Fig. 4: The uncharacterised protein YacL binds to RNA polymerase.
From: Reliable identification of protein-protein interactions by crosslinking mass spectrometry
a PPI subnetwork of RNAP. Line thickness between proteins (circles) increases with frequency of observed crosslinks (i.e. one, two, three and more crosslinked residue pairs). Colour scheme for RNAP binders: light grey = coelution correlation > 0.5, dark grey = STRING database combined score ≥ 150, black = both of the previous categories. b Elution traces of RNAP (average abundance of its subunits) and selected RNAP binders with their minimal elution correlation coefficient to any RNAP constituent. c Volcano plot showing the affinity enrichment of SPA-tagged YacL, which co-enriches RNAP (orange) and a number of proteins found crosslinked to the RNAP in the YacL affinity-enrichment experiment (violet). d RNAP with bound NusG (PDB 6C6U [10.2210/pdb6c6u/pdb]) and the region of Crosslinking MS-defined accessible interaction space with 14 satisfied restraints for YacL (I-TASSER model, placed for visualisation purposes) highlighted. Crosslinks between YacL and NusG or RNAP are highlighted in blue.
