Fig. 4: Transformation frequency at swarm boundaries is largely unaffected by DNA concentration.
From: Kin discrimination promotes horizontal gene transfer between unrelated strains in Bacillus subtilis
a DNA concentrations at the meeting points of self-controls (green), kin (blue) and non-kin strains (red), b DNA concentration at the swarm boundary between non-kin wt PS-216Sp and PS-196Cm (red) and between nuclease mutants (DNase mutants) PS-216Sp ΔyhcR ΔnucB and PS-196Cm ΔyhcR without exogenously added DNA (pink) (left panel) and with 30 µg DNA added at the swarm boundary (right panel, wt orange, DNase mutants yellow), asterisks represent statistically significant values compared to corresponding wt pairings (two tailed Student’s t-test for unpaired data assuming equal variances), c transformation frequency at the boundary of two non-kin wt (PS-216Sp and PS-196Cm) (red) and between two nuclease mutant strains (PS-216Sp ΔyhcR ΔnucB and PS-196Cm ΔyhcR) (pink), d transformation frequency at the boundary of two non-kin wt strains (PS-216Sp and PS-196 m) (red) and between two nuclease mutants strains (PS-216Sp ΔyhcR ΔnucB and PS-196Cm ΔyhcR) (pink) calculated per ng available DNA. Assays were replicated three times. See Table 2 for strain abbreviations. Data are presented as mean values ±SD and error bars represent SD of the mean values (n = 3). For statistical parameters see Supplementary Notes.
