Fig. 3: GTPBP6 binding causes rearrangements in the PTC.
From: Structural basis of GTPase-mediated mitochondrial ribosome biogenesis and recycling
a Cryo-EM structure of the GTPBP6-bound large mitoribosomal subunit (mtLSU) assembly intermediate (dataset 2). Depiction as in Fig. 1a with GTPBP6 in marine. b Structure of human GTPBP6. The protein is depicted schematically on top, with domain annotation indicated by coloring and residue numbers. The black bar above represents regions modeled in the structure. The density for the bound GTP is shown as indent. c, d Rearrangements in the peptidyl transferase center (PTC) upon GTPBP6 binding. The PTC region is shown enlarged in the MTERF4-NSUN4-GTPBP5 (c) and the GTPBP6 (d) mtLSU assembly intermediates. The rRNA and proteins are shown as cartoons, with coloring as in Fig. 2 and uL16m in ocher. NTD N-terminal domain, CTD C-terminal domain.
