Fig. 1: Cryo-EM structures of the human mitochondrial recycling complexes in three functional states.
From: Distinct mechanisms of the human mitoribosome recycling and antibiotic resistance
Segmented cryo-EM maps of the mitoribosomal a 55S•RRFmt complex (Class I), b 55S•RRFmt•EF-G2mt•GMPPCP complex (Class II), and c 39S•RRFmt•EF-G2mt•GMPPCP complex (Class III). In these three panels, the 28S subunit is shown in yellow, the 39S subunit in blue, E-tRNA in orchid, RRFmt in green and EF-G2mt in red. A lighter shade of yellow differentiates the 28S ribosomal proteins from the 12S rRNA, while a lighter shade of blue differentiates the 39S ribosomal proteins from the 16S rRNA. Landmarks of the 28S subunit: h, head; b, body. Landmarks of the 39S subunit: CP central protuberance, Sb stalk base; L1, MRP uL1m. d Cryo-EM density of RRFmt extracted from the Class I complex. e Cryo-EM density of RRFmt extracted from the Class III complex. f Cryo-EM density of EF-G2mt extracted from Class III complex. See Figs. S1 and S2, for overall and local resolutions, respectively, of densities corresponding to the mitoribosome, RRFmt, and EF-G2mt in these complexes. g Domain organization in RRFmt displaying domain I (light green), domain II (dark green), modeled region of NTE (pink) and unmodelled region of NTE (gray) h Domain organization in EF-G2mt showing G domain (orange), domain II (purple), domain III (cyan), domain IV (red) and domain V (blue).
