Table 1 Variants detected in the 5-FC resistance clones.

Clone name	Mutation Type	Nt change*	AA change	Gene symbol	functional annotation	Stability of 5-FC resistance	MIC (µg/ml) by MTS^TM
H99							0.023–0.032
HL1	Deletion	c.414GA>G		CNAG_02337	Fur1	stable	>32
HL4	Deletion	c.52AT>A		CNAG_00613	Fcy1	stable	>32
HL4	Substitution	c.898 C>A	H300N	CNAG_04784	Monosaccharide transporter	unstable	>32
HL8	Deletion	c.508GTTC>G		CNAG_02337	Fur1	unstable	>32
HL9	Substitution	c.120G>T	L40F	CNAG_00613	Fcy1	stable	>32
HS2	Substitution	c.235G>A	G79S	CNAG_05935	uridylate kinase	unstable	>32
R265							0.064–0.094
RL4	Substitution	c.952G>A	A318T	CNBG_9478	hypothetical protein	unstable	>32
RL4	Deletion	c.142GAAA>G		CNBG_1368	hypothetical	unstable	>32
RL4, RL5	Substitution	c.1549 G>A	A517T	CNBG_4985	hypothetical protein	unstable	>32
RL4, RS1, RS14	Deletion	c.540CTTTTTG>C		CNBG_4048	Fur1	un,st,st	>32
RL9, RS1, RS14, RS15	Deletion	c.75CTT>C		CNBG_4048	Fur2	us,st,st,st	>32
RL11	Deletion	c.1260CTA>C		CNBG_3227	Fcy2	stable	>32
RL11	Substitution	c.695T>C	M232T	CNBG_9545	hypothetical protein	stable	>32
RL12	Substitution	c.470 C>T	A157V	CNBG_3227	Fcy2	stable	>32
RS1	Substitution	c.569 C>T	S190F	CNBG_2198	cytoplasmic protein	stable	>32
WM276							0.016–0.023
WL1	Substitution	c.260 G>A	G87D	CGB_E2660C	Fur1	stable	>32
WL8	Deletion	c.829ATT>A		CGB_G3260C	Uxs1	unstable	>32
WL9	Deletion	c.538GTT>G		CGB_G3260C	Uxs1	unstable	>32
WL11	Deletion	c.460GAA>G		CGB_A6420C	Fcy1	stable	>32
WL15	Substitution	c.514G>T	V172L	CGB_F6195W	uridylate kinase	unstable	>32
WS13	Deletion	c.248GCT>G		CGB_E1620C	transposable element	unstable	>32
WS3	Deletion	c.456ATC>A		CGB_E2660C	Fur1	stable	>32
WS5	Substitution	c.1522GG>T	G508C	CGB_F1400C	epsilon DNA polymerase	unstable	>32

Quick links

Search