Fig. 4: Engineering of protein aggregates by combining Y15 self-assembly and protein–protein interactions.
From: Intracellular artificial supramolecules based on de novo designed Y15 peptides
a Schematic illustration of the assembly of Y15-tagged monomeric AG and tetrameric AG. b Fluorescence images of HEK293 cells expressing Y15-mAG-HA and tetrameric Y15-AG-HA. Scale bar, 10 µm. c Fluorescence anisotropy measurements of transfected HEK293 cells (n = 3 biologically independent experiments). Data are presented as mean values +/− SD. Source data are provided as a Source data file. d Correlative light and electron microscopy of Y15-AG expressing COS-7 cells, fluorescence image (left panel), overlay of the images (2nd and 3rd panels) and a higher magnification TEM image (right panel). Granular structures were not surrounded by membranes (arrowheads). e Fluorescence observation of co-transfected cells with Y15-AG-HA and Y15-mCherry-HA. Scale bar, 10 µm.
