Fig. 1: Cd36 expression in gut and mesenteric lymphatic vessels.
From: Visceral obesity and insulin resistance associate with CD36 deletion in lymphatic endothelial cells
a Representative staining for CD36 (green) and its colocalization with lymphatic vessel endothelial hyaluronan receptor 1, LYVE-1, (red) in lymphatic capillaries (lacteals) and in collecting vessels within the submucosa in C57BL/6 wild-type (WT) mice. Nuclei stained with DAPI (blue). Images were acquired using a Zeiss LSM 880 Airyscan Confocal microscope, ×20 and ×40 objective. Scale bar: 50 μm (top panel) and 10 μm (bottom and panel). b Flow cytometry to identify intestinal and mesenteric lymphatic endothelial cells (LECs) (CD45–CD90.2+CD31+). c CD36 expression in LECs isolated from gut mucosa (red) and submucosa (blue), and the mesentery (orange) in WT mice. Jejunum from Cd36−/− mice and IgG isotype control antibody used at the same concentration as CD36 antibody were included to document CD36 antibody specificity. Mice (n = 6) used in (a–c) are 12-week old. SSC side scatter, MFI mean fluorescence intensity, CD cluster of differentiation. Data are representative of three experiments with n representing the number of mice per group.
