Fig. 5: CDT1 is enriched at early origins.
CDT1 ChIP-seq was performed in HCT116 cells exposed to MLN4924 for 36 h (re-replication). a IGV screenshots of the distribution of CDT1 binding sites on parts of chromosome 4 and 19 in normally proliferating (control) and re-replicating cells. Input from control cells is shown at the top (for screenshot with input for both control and re-replication, see Supplementary Fig. 9). DNA replication timings of the genomic regions are shown at the bottom. b Heatmap summarizing the normalized sequencing coverage of CDT1 ChIP-seq in normally replicating control and re-replicating samples. CDT1 peaks called against input were first intersected with origins (CDT1 binding sites overlapping with origins), then were divided into 6 groups based on the replication timing data in Fig. 4. c Enrichment of CDT1 centered on replication origins residing in replication timing domains stratified as in b, was shown as violin plots. The box range is between 25th percentile and 75th percentile. The whisker length is defined as 1.5 * IQR (Interquartile range). Two biological repeats yielded similar results.
