Fig. 2: rTcpC inhibits NETosis.

a Confocal microscopy to detect the influence of rTcpC on ROS-dependent NETosis. Scale bar = 5 μm. b Percent of co-localization reflecting the NETosis levels in experiments as described in a. Mean ± SD of three independent experiments were shown. c FI of co-localization in experiments as described in a. b, c p < 0.01 was considered to be extremely significant. The FI reflecting NETosis level in the control group was set as 1.0. Mean ± SD of three independent experiments were shown. p < 0.01 was considered to be extremely significant. NS not significant. p-values were derived by Mann–Whitney multiple comparisons test. d Confocal analyses of the influence of rTcpC on ROS production. Scale bar = 25 μm. Images shown are representative of three independent experiments. n = 3. e FI of ROS were analyzed by ImageJ software. Mean ± SD of three independent experiments were shown. p < 0.01 was considered to be extremely significant. p-values were derived by Dunnett comparison test. f Flow cytometry detection of the effects of rTcpC on ROS production in LPS-induced NETosis. g, h Protein and mRNA levels of IL-1β, IL-6, TNF-α were detected by ELISA and qRT-PCR, respectively. Mean ± SD of three independent experiments (n = 3). p < 0.05 and p < 0.01 were considered to be statistically significant and extremely significant respectively. p-values were derived by Dunnett comparison test. Source data for panel b, c, e, g, h are provided in the separate Source Data file.