Fig. 3: rTcpc inhibits chromatin decondensation in NETosis. | Nature Communications

Fig. 3: rTcpc inhibits chromatin decondensation in NETosis.

From: TcpC inhibits neutrophil extracellular trap formation by enhancing ubiquitination mediated degradation of peptidylarginine deiminase 4

Fig. 3

a Dynamic analyses of chromatin decondensation by Sytoxgreen staining. Scale bar = 5 μm. b ImageJ analyses of DNA fluorescence area from experiments as described in a. a, b Mean ± SD are derived from three independent experiments in which five independent areas per condition were measured. p < 0.05 compared with LPS group, p-values were derived by Dunnett test. c ImageJ analyses of confocal images from experiments as described in a. Mean ± SD of three independent experiments were shown. p < 0.05 and p < 0.01 were considered to be statistically significant and extremely significant respectively, p-values were derived by Dunnett and Mann–Whitney tests. d Dynamic analyses of CitH3 in neutrophils with different treatment by western blot. e Gray scale analyses of bands from experiment as described in d. Mean ± SD of three independent experiments were shown, n = 3. p < 0.05 and p < 0.01 were considered to be statistically significant and extremely significant respectively, p-values were derived by Dunnett and Mann–Whitney tests. All western blots in panels d are provided as uncropped blots in Gels and Blots of Source Data file. Source data for panel b, c, e are provided in the separate Source Data file.

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