Fig. 1: Development of monobodies against CrSAS-6.
From: Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly
a CrSAS-6 homodimers (in blue) form ring polymers ~23 nm in diameter (left). Higher magnification views on the right show targets utilized for monobody selection: CrSAS-6_N (top) and CrSAS-6_6HR (bottom). b Monobody ribbon representation. The variable regions in the side-and-loop monobody library are colored: FG loop in red, side residues in connecting ßC/ßD strands in green and yellow, respectively, CD loop in blue. The amino acid sequences of the variable region for MBCRS6-1, MBCRS6-13 and MBCRS6-15 are shown on the right. c-e ITC profiles for the interaction between the targets CrSAS-6_6HR or CrSAS-6_N and the monobodies MBCRS6-1 (c), MBCRS6-13 (d), and MBCRS6-15 (e). f-h Structures of CrSAS-6_6HR (f) or CrSAS-6_N (g, h) in surface and ribbon representation (blue), highlighting in orange the residues interacting with MBCRS6-1 (f), MBCRS6-13 (g), and MBCRS6-15 (h), which are shown in gray in surface and ribbon representation, also in the higher magnifications on the right.
