Table 1 Efficient enrichment of mobile element signals in nanopore reads.

From: Cas9 targeted enrichment of mobile elements using nanopore sequencing

MEI

Run

Flow cell

Read number

On-target

Close-target

Off-target (%)

Reference (%)

Non-reference (%)

Reference (%)

 

Individual

 L1Hs

ABB607

Flongle

4102

49.6

7.3

16.8

26.3

 AluYb

ACK645

2271

40.2

6.5

1.0

52.2

 AluYa

ACK655

12,513

18.0

5.8

10.3

65.9

 SVA_F

ACK629

14,106

3.7

2.1

3.7

90.6

 SVA_E

ABO395

7297

1.7

0.6

0.2

97.5

 L1Hs

FAL11389

MinION

110,029

30.7

4.3

37.9

27.1

Pooled

FAO84736

MinION

105,410

20.1

3.2

38.4

38.3

 L1Hs

   

8.9

1.6

33.9

 

 AluYb

   

7.0

1.2

3.2

 

 AluYa

   

2.8

   

 SVA_F

   

1.2

0.4

1.3

 

 SVA_E

   

0.2

   
  1. Summary of seven representative flow cells: five individual Flongle flow cells for L1Hs (ABB607), AluYb (ACK645), AluYa (ACK655), SVA_F (ACK629), and SVA_E (ABO395) each, one individual MinION flow cell for L1Hs (FAL11389), and one pooled MinION flow cell for five MEIs (FAO84736).