Fig. 2: IL-6 promotes autophagy in a JAK2 signaling-dependent manner.

a Western blotting was performed for SW48 and LoVo cells separately treated with IL-6 (20 ng/ml) in the absence or presence of CHZ868 (0.2 μM) for 12 h (n = 2 independent experiments). b SW48 and LoVo cells stably expressing the GFP-LC3B fusion protein were separately treated with IL-6 (20 ng/ml) in the absence or presence of CHZ868 (0.2 μM) for 12 h. Confocal microscopy images are shown. Scale bars, 20 μm (n = 3 independent experiments). c SW48 and LoVo cells were separately transfected with the JAK2 or control plasmid. After transfection for 24 h, cells were treated with IL-6 (20 ng/ml) for 12 h. Western blotting was performed to examine the expression of LC3B-II and SQSTM1 (n = 2 independent experiments). d SW48 and LoVo cells stably expressing the GFP-LC3B fusion protein were separately transfected the JAK2 or control plasmid, and after stimulation with IL-6 (20 ng/ml) for 12 h, confocal microscopy images were obtained to measure the number of GFP puncta. Scale bars, 20 μm (n = 3 independent experiments). e SW48 and LoVo cells were separately transfected with small interfering RNA targeting JAK2 (SiJAK2) or small interfering RNA targeting a negative control gene (SiNC). After transfection for 24 h and following stimulation with IL-6 (20 ng/ml) for 24 h, western blotting was performed to examine the expression of LC3B-II and SQSTM1 (n = 2 independent experiments). f SW48 and LoVo cells stably expressing GFP-LC3B fusion protein were separately transfected with SiJAK2 and SiNC. After stimulation with IL-6 (20 ng/ml) for 24 h, confocal microscopy images were obtained to measure the number of GFP puncta. Scale bars, 20 μm (n = 3 independent experiments). Source data are provided in the Source Data file.