Fig. 2: Super-resolution three-dimensional VISTA imaging of cells and tissues.
From: Super-resolution label-free volumetric vibrational imaging
a–d Mitotic HeLa cells in prophase (a), metaphase (b), anaphase (c), and telophase (d). e–g Zebrafish embryonic retina before processing and after expansion: unprocessed (e), expanded (f), and the zoom-in view from the boxed region in (f, g). h A mosaic VISTA image of hippocampal tissue. i–l zoomed-in high-resolution view from color-boxed areas (i red box; j blue box) and selected regions in (h). Representative neuronal cell bodies, neuronal processes, and likely blood vessel cross-sections are indicated by green, blue, and white arrows, respectively in (j). m 3D volume VISTA imaging of a hippocampal tissue throughout 1000 μm depth. Scale bars: 20 μm (a–g) and 200 μm (h–m). For processed samples, the length scale is in terms of distance after expansion.
