Fig. 3: Validation of VISTA imaging features with fluorescent markers on mouse brain tissues.
From: Super-resolution label-free volumetric vibrational imaging
a–d Parallel images of VISTA (a, c) and fluorescence from lectin-DyLight594 stained blood-vessel (b, d) in vessel-abundant regions. e–g Parallel images of VISTA (e) with two-color fluorescence from lectin-DyLight594 stained vessels (f) and DAPI-stained nuclei (g). h Three-channel merged image from (e–g). i, j Parallel images of VISTA (i) and fluorescence from immuno-stained NeuN, the matured neuron marker (j). k, l Parallel images of VISTA (k) and fluorescence from immuno-stained MAP2, the neuronal cell body, and dendrite marker (l). All images are shown as maximum intensity projection from a stack of volume images. Scale bars: 40 μm. The length scale is in terms of distance after sample expansion.
