Fig. 2: Validation of ²H enrichment in palmitate and body water.

a High-resolution M + 1 mass spectra of [1-²H₁]palmitate enrichment standards. b Calibration curve of [1-²H₁]palmitate enrichment standards by full scan acquisition (each point measured in triplicate). c Natural abundance MID acquired by t-SIM at different AGC targets. d Calibration curve of ²H-palmitate enrichment standards by t-SIM acquisition at different AGC targets. e Expansion of curve for low enrichment samples. f High resolution M + 1 mass spectra of ²H-acetone enrichment standards generated by using various ²H₂O enrichments. g Calibration curve of ²H-acetone enrichment standards by t-SIM acquisition. h Expansion of curve for low enrichment samples. i Evolution of plasma body water enrichment with different ²H₂O doses in mice (n = 5 mice per dose except for the 20 μl/g dose which had n = 4). j Plasma ²H₂O enrichment as a function of ²H₂O dose in mice (n = 5 mice per dose except for the 20 μl/g dose which had n = 4). Where appropriate Mean and SEM are shown and R² determined by simple linear regression.