Fig. 3: 2H2O administration and serial measurements of lipid synthesis in mice.
From: Measurement of lipogenic flux by deuterium resolved mass spectrometry
High resolution (a) M + 1 and (b) M + 2 mass spectra of 2H-palmitate from the plasma TG of a mouse after a 5 µl/g dose of 2H2O. c Palmitate enrichment kinetics measured after different 2H2O doses (n = 5 mice per dose for all doses except the 3-h time point which had n = 4). d Contributions of 2H1, 2H2 and 2H3 to palmitate enrichment after 24-h at different 2H2O doses (n = 5 mice per dose). Contributions calculated as 1 x 2H1, 2 x 2H2, and 3 x 2H3 fractional isotopomer distributions, as described in the Methods section. e Initial DNL rates were independent of 2H2O dose (no significant differences detected by 2-way Anova, n = 5 mice per dose except for the 20 μl/g dose which had n = 4). f Correlation between plasma TG palmitate and liver TG palmitate 24-h after various 2H2O doses (n = 5 mice per dose, total of n = 20 mice in the correlation). Mean and SEM shown where appropriate.
