Fig. 2: Optimization of the PAM-interacting domain to improve the editing efficiency of ABE-NG at the NG sites.
From: Efficient precise in vivo base editing in adult dystrophic mice
a–e Measurements of the base editing efficiencies of different ABE variants on five different sites with NG PAM. The gRNA sequences are listed on the top of each graph with the PAM sequences in red and the target adenines in blue. ns not significant; ****p < 0.0001; one-way ANOVA with Turkey’s multiple comparisons test to compare with the ABE-NG group. n = 3 wells/group. Data are mean ± SD. f Comparison of the editing efficiencies of different ABE variants at all five NG sites. n = 5 different sites/group; average of three different wells per site; repeated measures one-way ANOVA with Turkey’s multiple comparisons test. Data are mean ± SD.
