Fig. 3: Re-engineering the adenine deaminase domain to improve the efficiency and specificity of ABE-NG.
From: Efficient precise in vivo base editing in adult dystrophic mice
a Schematics of the adenine deaminase domain used in ABE-NG editors. b Quantification of the editing efficiency of different ABE-NG variants with modified TadA* domain at the mdx4cv target site. n = 6 wells/group; ****p < 0.0001; one-way ANOVA with Turkey’s multiple comparisons test. Data are mean ± SD. c The number of off-target RNA editing events in Neuro-2a cells transfected with different ABE-NG variants. n = 3 wells/group; one-way ANOVA with Turkey’s multiple comparisons test. Data are mean ± s.e.m. d Quantification of the off-target RNA editing (A-to-I) activities on four RNA adenines previously identified as being efficiently modified by ABEmax in HEK293 cells. n = 3 wells/group; ****p < 0.0001; one-way ANOVA with Turkey’s multiple comparisons test. Data are mean ± SD.
