Fig. 4: cPKA and NbSmo2 recruitment to SMO.

a Schematic presentation of the cPKA recruitment assay measuring direct BRET. b cPKA-YFP recruitment to SMO wild-type (black) and mutant (dark gray). CD86 (light gray) was employed as a negative control. c Scheme of the NbSmo2 recruitment assay measuring direct BRET. d NbSmo2-YFP recruitment to SMO wild-type (black) and mutant (dark gray). CD86 (light gray) was employed as a negative control. Experiments shown in b and d were conducted in ΔSMO HEK293 cells transiently co-transfected with the indicated receptor-Nluc construct and varying amounts of cPKA-YFP (b) or NbSmo2-YFP (d), respectively. Data show mean ± SD from four individual experiments conducted in quadruplicates. Each dataset was fitted to a one-phase association or linear model and the preferred fit was selected based on an Extra sum-of-squares F-test (P < 0.05). See Supplementary Fig. 6f for the cell-surface expression data of the receptor-Nluc constructs. Source data are provided as a Source Data file.