Fig. 5: H-1PV directly binds to laminins through sialic acid presumably within the heparin-binding site.

a H-1PV binds to laminins in ELISA. Selected wells of a 96-well microtiter plate were pre-coated with purified LN211, LN221, LN411, LN421, LN332, collagen type IV, fibronectin and BSA. After blocking non-specific binding, wells were treated with H-1PV or left untreated, followed by stringent washing to remove loosely bound virus particles. The bound virus particles were detected by ELISA using a virus capsid conformational antibody as described in the M&M. The independent experiment shown is repeated twice; n = 2. b H-1PV binds to laminin via sialic acid moieties. Wells pre-coated with purified LN411, LN421 or BSA were treated or not with NA. The bound virus particles were detected by ELISA as described in Fig. 5a. The independent experiment shown is repeated twice; n = 2. c Treatment with heparin decreases H-1PV-interaction with laminins without binding to the virus capsid. Selected wells of a 96-well microtiter plate were pre-coated with purified LN421, BSA-conjugated heparin or BSA. The wells with applied LN421 or BSA were pre-treated with heparin or NA or left untreated. ELISA was performed as above. Each column represents the mean value ± the standard deviation (n = 2). The independent experiment shown is repeated twice; n = 2. Error bars for all data indicate the mean values ± SD. Source data are provided as Source Data file.