Fig. 2: FYN-TRAF3IP2-dependent signaling intersects with TCR signaling.
a Outgrowth of Ba/F3 cells transduced with empty pMIG vector, pMIG-FYN1–232, pMIG-TRAF3IP2 or pMIG-FYN-TRAF3IP2 after withdrawal of IL-3. n = 3 biological replicates per condition. b qRT-PCR for IL-17-regulated transcripts in naive CD4+ T cells transduced with empty pMIG vector, pMIG-TRAF3IP2 or pMIG-FYN-TRAF3IP2. n = 3 biological replicates per condition. p values were calculated with Tukey’s post-hoc multiple comparisons test. c Western blot analysis for canonical and non-canonical NF-κB activation in Jurkat cells transduced with empty pMIG vector, pMIG-TRAF3IP2 or pMIG-FYN-TRAF3IP2. d NF-κB GFP reporter signal intensity in Jurkat cells with empty control vector or expression of FYN1–232, TRAF3IP2 or FYN-TRAF3IP2 after stimulation with an agonistic anti-CD3ε antibody or exposure to control immunoglobulin (Ig). n = 3 biological replicates per condition for Ig control, n = 4 for anti-CD3ε. p values were calculated with Tukey’s post-hoc multiple comparisons test. e Western blot analysis for activation of MAPK pathways in Jurkat cells transduced with empty pMIG vector, pMIG-TRAF3IP2 or pMIG-FYN-TRAF3IP2. f Intracellular flow cytometry for IκBa (top row) or phospho-p65 (bottom row) in CD4+ T cells treated with control immunoglobulin (Ig) or agonistic anti-CD3e antibody. n = 3 biological replicates per condition. p values were calculated with Šidák’s multiple comparisons test (two-sided). g Intracellular flow cytometry proximal TCR signaling and MAPK pathway activation in CD4+ T cells treated with control immunoglobulin (Ig) or agonistic anti-CD3e antibody. n = 3 biological replicates per condition. p values were calculated with Šidák’s multiple comparisons test (two-sided). All data are represented as mean ± standard deviation (SD).
