Fig. 5: Trans silencing within the germline can be followed by recovery of gene expression and resistance to subsequent silencing within a few generations.
From: Mating can initiate stable RNA silencing that overcomes epigenetic recovery
a Tcherry or Tcherry∆pi animals were mated with iT stably silenced for >150 generations and fractions of animals with bright Tcherry or Tcherry∆pi expression were scored in resulting cross progeny (F1) and their descendants (F2 through ≤F8). Error bars indicate 95% confidence intervals. Schematics of transgenes used are indicated above the graphs. See Supplementary Fig. 7j,k for number of animals analyzed in each generation for each genotype and for pedigree. Fractions of bright animals are plotted and error bars indicate 95% CI. b pgl-1::gfp animals were mated with non-transgenic or iT animals and fluorescence of PGL-1::GFP was quantified in cross progeny (left) or scored as in Fig. 1c in cross progeny and their descendants (middle and right). Chromosomes with a recessive dpy marker (blue font) and number of animals scored (n) are indicated. Also see Supplementary Fig. 7. Asterisks indicate P < 0.05 using two-tailed Student’s t-test (b, left) or χ2 test (b, right). Source data are provided as a Source Data file.
