Fig. 10: PR function but not RPE pathology can be rescued by exogenous retinoid.

a Schematic of the visual cycle and exogenous 9-cis retinal treatment. b Eight-to-nine-months-old Cib2-mutant mice (Cib2^KO/+ and Cib2^KO/KO) were either intraperitoneally injected with vehicle (n = 7) or 0.25 mg 9-cis retinal (n = 8), dark-adapted overnight, and ERG performed the next day. Both a-wave (left panel) and b-wave (right panel) amplitudes were higher in mutant mice injected with 9-cis retinal than vehicle-injected mice. For comparison, untreated WT control mice (black squares, n = 5) are replotted from Fig. 1c, bottom panels. c Schematic of treatment paradigm in RPE-specific Cib2 mutant mice injected with 9-cis retinal and control mice injected with vehicle. d Baseline ERG for indicated genotype is reproduced from Fig. 2a, bottom panels. e ERG amplitudes for indicated genotype after specific treatment as outlined in c (vehicle-injected; n = 6, 9-cis retinal injected n = 7). f TEM micrographs for indicated genotype at the end of the treatment paradigm as outlined in c (n = 3/genotype). Scale bar, 2 µm. Data presented as mean ± SEM; each data point represents an individual animal. Unpaired two-tailed t-test, p < 0.05 (*).