Fig. 2: NDF5 formed a putative protein complex depending on PnsB2, PnsB3, and Lhca6. | Nature Communications

Fig. 2: NDF5 formed a putative protein complex depending on PnsB2, PnsB3, and Lhca6.

From: Evolution of an assembly factor-based subunit contributed to a novel NDH-PSI supercomplex formation in chloroplasts

Fig. 2

Protein complexes of the chloroplast membrane isolated from Arabidopsis wild-type (AtWT) (a) and SubB and lhca6 mutants ((b) and (c)) were separated using SDG ultracentrifugation. The top of the centrifugation tube is at the left. After centrifugation, the SDG was divided into 30 fractions from top to bottom, and the fractions subjected to immunoblot analysis. The centrifugation tube of the AtWT is shown as a representative pattern on the top in (a). The centrifugation tube and blotting patterns were fitted according to Coomassie Brilliant Blue-stained gels (Supplementary Fig. 2b). The positions of the NDH-PSI supercomplex and PSI-LHCI are indicated with black arrows. Asterisks indicate non-specific signals. Each experiment was performed at least twice.

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