Fig. 6: Gene X in Physcomitrella can complement the Arabidopsis ndf5 mutant. | Nature Communications

Fig. 6: Gene X in Physcomitrella can complement the Arabidopsis ndf5 mutant.

From: Evolution of an assembly factor-based subunit contributed to a novel NDH-PSI supercomplex formation in chloroplasts

Fig. 6

a Transient increases in chlorophyll fluorescent were monitored after turning off AL in Arabidopsis wild-type (AtWT), ndf5, pnsb2, 35Sp::Gene X/ndf5, and 35Sp::Gene X/pnsb2, as in Fig. 5b. A typical trace of chlorophyll fluorescence in the AtWT and transient increases in chlorophyll fluorescence (boxed area) are shown at left and right, respectively. b The expression level of Gene X in AtWT, mutants, and transgenic plants, as analyzed by RT-qPCR. c Separation of photosynthetic protein complexes by BN-PAGE. The gel was stained with Coomassie Brilliant Blue (right panel). Each band was identified according to previous work71 and is indicated by black arrows at left. The top part of the BN gel is magnified at right. d Immunoblot analysis of chloroplast membrane proteins isolated from AtWT, mutants, and transgenic plants. Sample loading was based on chlorophyll content. Cytf was detected as a loading control. This is a result of a single experiment using a T1 plant. The results using independent T1 plants are also shown in Supplementary Fig. 5.

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