Fig. 8: The NDH activity was not affected in the Gene Y knockout mutant in Physcomitrella. | Nature Communications

Fig. 8: The NDH activity was not affected in the Gene Y knockout mutant in Physcomitrella.

From: Evolution of an assembly factor-based subunit contributed to a novel NDH-PSI supercomplex formation in chloroplasts

Fig. 8

a RT-PCR analysis of Gene Y and PnsB4 transcripts. These transcripts were amplified using cDNA from Physcomitrella wild-type (PpWT) and Gene Y knockout mutants (Gene Y_KO). PnsB4 was detected as a control. b Analysis of transient increases in chlorophyll fluorescent after turning off actinic light (AL), as in Fig. 5b. A typical trace of chlorophyll fluorescent in the PpWT and the transient increases in chlorophyll fluorescent (boxed area) are shown at left and right, respectively. c Immunoblot analysis of membrane proteins from PpWT and Gene Y_KO #10. Samples were loaded on the basis of chlorophyll content, and PnsB1 and PsaA (a loading control) were detected. Each experiment was performed at least twice, and results in independent transgenic plants are also shown in Supplementary Fig. 11d.

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