Fig. 4: UPF1 stimulates repair of non-telomeric DNA damage.
From: UPF1 promotes the formation of R loops to stimulate DNA double-strand break repair
a Western blot showing the levels of the indicated proteins in RPE1 WT or UPF1 KO cells treated with bleomycin (10 µg/ml) for 90 min. b Quantification of western blot showing activation of DNA damage response proteins in RPE1 WT or UPF1 KO cells treated with bleomycin (10 µg/ml) for 90 min. The values of phosphorylated proteins shown are relative to the values in WT and normalized with the level of total proteins. Data plotted are means (n = 2). c, d Cell cycle analysis of RPE1 WT or UPF1 KO cells untreated or treated with 50 µg/ml bleomycin for 3 days (c) with the quantification of the level of sub-G1, G1, S and G2/M cells (d). Data plotted are mean + SEM (n = 3 for WT and UPF1 46, n = 2 for UPF1 54). p values were obtained using Student’s t-test (unpaired two-tailed, equal variance). e–g Analysis of the efficiencies of single strand annealing (e), homologous recombination (f), and End-joining (g) in U2OS WT cells transfected with UPF1 or control siRNAs40. Data plotted are means + SEM (n = 4 for e, n = 3 for f, g). p values were obtained using Student’s t-test (unpaired two-tailed, equal variance). h Analysis of NHEJ activity in RPE1 WT or UPF1 KO cells. Data plotted are means + SEM (n = 3). p values were obtained using Student’s t-test (unpaired two-tailed, equal variance). The n values indicate the number of independent experiments in all cases.
