Fig. 6: UPF1 stimulates R loop formation at sub-telomeric DSBs.
From: UPF1 promotes the formation of R loops to stimulate DNA double-strand break repair
a–c QAOS analysis showing the level of 3′ and 5′ ssDNA at the indicated loci in RPE1 WT cells at the indicated time after TALEN nucleofection (a, b) or RPE1 cells expressing RNaseH1 or GFP at 10 h after nucleofection (c). Data plotted are mean + SEM, n = 5 for a, n = 5 for b (except for 5 h, n = 4), n = 3 for c. p values were obtained as described in Fig. 3a. d DRIP analysis showing the levels of DNA–RNA hybrids at the indicted loci in RPE1 cells transfected with CtIP or control siRNAs at 10 h after nucleofection. Data plotted are mean + SEM (n = 3). p values were obtained as described in Fig. 5c. e, f QAOS analysis in RPE1 cells transfected with CtIP or control siRNAs (e) and RPE1 WT and UPF1 KO cells (f) at 10 h after nucleofection. Data plotted are mean + SEM (n = 3). p values were obtained as described in Fig. 3a. The n values indicate the number of independent experiments in all cases.
