Fig. 4: In vitro assay of Treg cell paths.
a−c Flow cytometry and its quantification of the indicated proteins in each HD PB Treg cell path (CD25: n = 3, CTLA4: n = 3, IL10: n = 4, TGF-β1: n = 3, GZMA: n = 3, GZMB: n = 3, LAP: n = 3). d The proliferation of HD PB Treg cell paths. The Tag-it Violet dilution of Treg cells were assessed by flow cytometry, after 96 h in vitro culture (n = 3). e In vitro suppression assay (n = 3). Tag-it Violet-labeled HD PB Tresp cells were co-cultured with different HD PB Treg cell paths for 96 h with CD3/CD28 T cell activator. The Treg: Tresp ratio was 1: 2. The Tag-it Violet dilution of Tresp cells were assessed by flow cytometry. In a–e, experiment repeated at least three times; p values were determined by One-way ANOVA; data are presented as mean values ± SEM. Source data are provided as a Source Data file.
