Fig. 9: ATR deletion changes location and enrichment of SYT2.
From: ATR regulates neuronal activity by modulating presynaptic firing
a Sagittal sections from 3-month-old control (Ctr) and ATR-FBΔ (FBΔ) mice were stained with DAPI (blue), SYT1 (green) and SYT2 (red). The dentate gyrus area is shown. GL Granular Layer, H Hilus. b Immunofluorescence analysis of expression patterns of SYT1 and SYT2. Note a high SYT2 expression in granule cells in the GCL in controls, but more abundant and co-localization with SYT1 in mutant hilus (zoom-in pictures on right). c Sagittal sections from 3-month-old control and ATR-FBΔ mice were stained with DAPI (white), SYT2 (red) and VGAT (green). CA: Cornu Ammonis, DG: Dentate Gyrus). d Immunofluorescence analysis of expression pattern of VGAT and SYT2. Note a colocalization of VGAT and SYT2 in granule cells in the GCL in controls, but more abundant, yet not overlapping with VGAT, in mutant hilus (zoom-in pictures on right). e Sagittal sections from 3-month-old control and ATR-FBΔ mice were stained with DAPI (blue), SYT2 (red) and VGLUT1 (green). CA Cornu Ammonis, DG Dentate Gyrus. f Immunofluorescence analysis of expression pattern of VGLUT1 and SYT2. Note a high VGLUT1 in the hilus, where the SYT2 expression is low, in controls. Strong overexpression and co-localization (puncta) of VGLUT1 and SYT2 in the hilus (right zoom-in pictures). The images are representative from 4 to 5 mice per genotype analyzed.
