Table 1 Reaction optimization and control experiments^a.


Entry	Deviation from standard conditions	Yield (%)^b	D-incorporation (%)^d
1	Standard conditions	99 (75^c)	93
2	Free FAD	0	–
3	No CvFAP	0	–
4	No light	0	–
5	No DMSO	17	93
6	CH₃CN instead of DMSO	96	95
7	H₂O instead of D₂O	99	0
8	Air	99	93

^aReaction conditions: Substrate (0.40 mmol), crude enzyme powder (containing CvFAP about 20 mg), D₂O (4 mL), DMSO (1 mL), 450 nm LED, 20 °C, 12 h.
^bYields were determined by GC.
^cIsolated yield.
^dD-incorporation are determined by ¹H NMR or HRMS.

Quick links

Search