Fig. 2: Characteristics of NSF/Sli in rendering SC to self-incompatible diploid potatoes.

a Collinearity analysis of the distal ends of chromosome 12 between RH and M6. Orthologous relations between the different syntenic loci are depicted by gray. The NSF gene is identified with the red arrow. b The number of self-compatible diploid potatoes induced by NSF/Sli. Six groups (S. stenotomum, S. goniocalyx, S. tuberosom, S. phureja, S. ajanhuiri, and S. curtilobum) and two subgroups (tuberosum and andigena) are indicated with different colors. The number represents the accessions that transfer from SI to SC after crossing with RH or E172. c Expression patterns of Sli in different developmental stages of RH flowers. Development of the RH flower was divided into five stages, including four developing stages according to bud size (status of each stage was shown as illustration) and one mature stage. Expression level was normalized to the expression in stage 1. StEF1-α was set as an endogenous reference gene. Data are presented as means ± s. d. (n = 3 biological replicates). d In situ mRNA hybridization of Sli in the stage 4 flower bud and mature stamen. d1 and d4, the stage 4 flower bud (d1) and mature pollen (d4) used in the in situ hybridization assay. d2 and d5, the transverse sections of the stage 4 flower bud (d2) and mature stamen (d5). Bar 200 μm. Magnification of the red square in d2 and d5 are presented in d3 and d6, respectively (bar is 20 μm). The experiments were repeated three times with similar results.