Fig. 3: LAIR1 targets a conserved interface within a subset of RIFIN family members.
From: Structural basis of LAIR1 targeting by polymorphic Plasmodium RIFINs
a Phylogenic analysis of plasmodium strain 3D7 RIFIN-v2 apex identified two additional RIFINs in the same branch as known LAIR1-binding RIFINs. b Co-IP assay confirmed the MGD21.LAIR1-binding by the two new RIFINs. The SDS-PAGE runs were repeated at least twice. c Superimposition of two RIFIN-LAIR1 complex structures. d Sequence comparison of MGD21-positive (cyan and magenta) and MGD21-negative (green) RIFINs V2 region, with LAIR1-binding region highlighted in red and conserved residues near the binding region marked with asterisks (*). e Co-IP with Alanine mutants in RIFIN-V2 four conserved residues. wt: wildtype PF3D7_1040300, m1: D247A, m2: C254A, m3: C265A, m4: R268A. The SDS-PAGE runs were repeated at least twice.
