Fig. 3: FG phases stained by fluorescent aromatic compounds.
From: Recapitulation of selective nuclear import and export with a perfectly repeated 12mer GLFG peptide
a Mac98A FG particles were formed, incubated with 2 µM of indicated fluorophores, and analyzed by CLSM. Note that all fluorophores got attracted by the phase; however, there were great differences with Atto488 being nearly inert and Cy3 accumulating very strongly. Table 1 lists all fluorophores tested and their partition coefficients. Chemical structures are shown in Supplementary Fig. 3. * indicates that a cysteine-quenched fluorophore maleimide was tested. Blue numbers: ratios of signals inside Mac98A FG phase to signal in the surrounding buffer. Scan settings/image brightness were adjusted individually. b scNup116 FG particles were challenged with efGFP8Q, or variants with an added C-terminal cysteine that had been modified either with maleimidopropionic acid (“Mal-quenched”), Atto488-, Alexa647-, Atto647N-, and Cy3-maleimides, respectively. Blue numbers: ratios of GFP fluorescence inside: outside the Nup116 FG phase. The far-red channel detects signals from Alexa647/Atto647N (colored cyan), and the red channel detects signals from Cy3 (colored magenta). Scanning settings/image brightness were adjusted individually. Note that, e.g., the Atto647N modification increased the GFP partition coefficient ≈30-fold.
