Fig. 9: Recapitulation of RanGTPase-controlled nuclear export with the FG phase formed by the perfectly repetitive variant.
From: Recapitulation of selective nuclear import and export with a perfectly repeated 12mer GLFG peptide
a The FG phase of prf.GLFG52x12 was incubated for 60 min with NES-EGFP, an ATP/GTP-regenerating system, and either (i) buffer only, (ii) Xpo1, (iii) Xpo1 and wild-type RanGTP (preloaded with GTP by RanGEF), or (iv) Xpo1 and RanGDP (converted by RanGAP to the GDP form). b In another setup, the FG phase of prf.GLFG52x12 was initially filled with the Xpo1·RanGTP·NES-EGFP complex in the presence of RanGEF, then incubated individually for 60 min with the indicated components, and finally imaged. c, d As in (b), the FG phase of prf.GLFG52x12 was initially filled with the Xpo1·RanGTP·NES-EGFP complex. At time=0 s, RanGAP and RanBP1 were added to trigger NES-EGFP efflux. The signal of GFP was recorded over time and analyzed as described in Fig. 8. Source data are provided as a Source Data file. d A plot of GFP signals inside FG particles of different radii against time and best-fits of the single exponential decay function: \(f\left(t\right)=A{e}^{-{kt}}+B\) (see also Supplementary Fig. 9). e Illustration of the above processes.
