Table 1 Mac98A/Nup116 FG phases stained by fluorescent aromatic compounds (complete list).
From: Recapitulation of selective nuclear import and export with a perfectly repeated 12mer GLFG peptide
Environmentally insensitive fluorophores | TtMac98A | scNup116 |
|---|---|---|
FG in:out | FG in:out | |
Abberior STAR 635P* | 16 | N.D. |
Alexa488* | 6.3 | 36 |
Alexa568* | 9.2 | 11 |
Alexa594* | 21 | 25 |
Alexa647* | 8.5 | 13 |
Atto488* | 2.0 | 2.0 |
Atto565* | 51 | 47 |
Atto647N* | 60 | 110 |
Cy3* | 110 | 610 |
Cy5* | 88 | 180 |
DyLight405* | 16 | N.D. |
DyLight488* | 3.7 | N.D. |
Fluorescein | 7.4 | N.D. |
Fluorescein* | 8.7 | 10.3 |
OregonGreen488* | 12 | N.D. |
Rhodamine B | 37 | 110 |
Sulfo-Cy3* | 66 | 130 |
Sulfo-Cy5* | 34 | 28 |
Environmentally sensitive fluorophores | TtMac98A | scNup116 |
|---|---|---|
FG in:out | FG in:out | |
DAPI | 1700 | 8100 |
Ethidium bromide | 22 | N.D. |
Hoechst 33258 | 3300 | N.D. |
Hoechst 33342 | 3300 | >10000 |
Hoechst 34580 | >10000 | >10000 |
Nuclear yellow | 970 | N.D. |
Oxazole yellow | 380 | N.D. |
SYPRO orange | 1000 | 1090 |
Thioflavin-T | 4.3 | 220 |
