Fig. 1: Imaging observations of diazotrophs in Tara Oceans samples.
From: Global distribution patterns of marine nitrogen-fixers by imaging and molecular methods
Images were obtained by environmental high content fluorescence microscopy (eHCFM; ref. 30), with the exception of Trichodesmium colonies, which were detected in situ using an Underwater Vision Profiler 5 (UVP5; ref. 33). From left to right, the displayed channels for each micrograph correspond to: cell surface (cyan, AlexaFluor 546), cellular membranes (green, DiOC6), chlorophyll autofluorescence (red), the bright field, and the merged channels. The displayed Hemiaulus-Richelia association was detected at station TARA_80 in the South Atlantic Ocean, Rhizosolenia-Richelia at TARA_53 in the Indian Ocean, Chaetoceros-Calothrix at TARA_131 (ALOHA) in the North Pacific Ocean, Climacodium-Croscophaera at TARA_140 in the North Pacific Ocean, the Croscophaera-like colony at TARA_53 in the Indian Ocean, the Trichodesmium filament at TARA_42 in the Indian Ocean, and the Trichodesmium colonies at TARA_141 and TARA_142 in the North Atlantic Ocean. Each micrograph is representative of the following number of images obtained in the current study: 350 Hemiaulus-Richelia, 56 Rhizosolenia-Richelia, 8 Chaetoceros-Calothrix, 1 Climacodium-Crocosphaera, 150 Crocosphaera-like colonies, 605 Trichodesmium-free filaments, 115 puff and 95 tuft colonies of Trichodesmium.
