Fig. 7: Knockdown of M1BP alters nuclear organization of insulator bodies.
a Epifluorescence imaging of insulator body localization using anti-CP190 in whole-mount brain, eye, leg, or wing imaginal disc tissues. M1BP knockdown is driven by Act5C-Gal4 driver. Insets show zoom of single nucleus outlined with dashed line in larger panel. Scale bars: 5 μm. Immunostaining experiments were performed three times with similar results. b Histograms showing the number of insulator bodies per nucleus in the experiment exemplified in (a). In all tissues, the number of insulator bodies is statistically significantly increased in M1BP knockdown (Kruskal–Wallis test; all Benjamini–Hochberg corrected P < 5 × 10–17, n = 100). c Area measurements of individual insulator bodies in brain, eye, leg, and wing imaginal disc tissues of control and M1BP knockdown larvae are shown. Bodies were measured (Tukey plots with outliers omitted, Mann–Whitney test P < 0.001, n = 200). d Area measurements of total insulator bodies per nucleus are shown (Tukey plots with outliers omitted, Mann–Whitney test P < 0.001); Brain: (Control n = 108, M1BPRNAi n = 109), Eye: (Control n = 121, M1BPRNAi n = 168), Leg: (Control n = 107, M1BPRNAi n = 103), Wing: (Control n = 109, M1BPRNAi n = 109). Note that not all cells have discernible nuclear demarcations. c, d Data are presented as boxplots where box represents the 25–75th percentiles and middle line is the median. The upper whisker extends from the hinge to the largest value no further than 1.5 × IQR from the hinge (where IQR is the interquartile range), and the lower whisker extends from the hinge to the smallest value at most 1.5 × IQR of the hinge, while data beyond the end of the whiskers are outlying points that are omitted from the plots.
