Fig. 3: Long-range distance inside the PRD of N- and T-fibrils is highly similar.
From: Huntingtin fibrils with different toxicity, structure, and seeding potential can be interconverted
a Sequence of the PRD indicating the positions of the labels introduced for distance measurements in red and the distance pairs as arrows. The segments of the PRD including the two polyP regions (P11 and P10) and the linker region (L17) and C-terminals region (C12) are also indicated. b Table listing all distances measured via EPR DEER experiment for each spin label pair and fibril type. The distance corresponds to the maximum of the main peak derived from the DEER decay curve (DEER data and fits are shown in Supplementary Fig. 2). In addition, the theoretical distances for a polyP II helix assuming an increase of 3.1 Å per residue and the theoretical radius of gyration (RG) for a random coil, are given (RG = R0 Nν with R0 = 1.927 Å, ν = 0.598, and N is the number of residues). Both distances spanning the polyP regions (P11 and P10) correspond nicely to a PPII distance. The distance spanning the L17 region is significantly shorter than a PPII helix and longer than expected for a random coil structure.
