Fig. 4: PolyP and HTTex1 difference spectra suggest an increased entanglement of the PRDs in N compared to T-fibrils.
From: Huntingtin fibrils with different toxicity, structure, and seeding potential can be interconverted
a CD spectra of polyPro at a concentration of 0.1 mg/ml recorded at 25 °C (black) and 90 °C (blue). The spectrum in red was recorded after the spectrum at 90 °C and after removing the liquid from the CD cuvette indicating the formation of a polyP film inside the cuvette. b Difference of N- and T-fibril CD spectra of Fig. 1 (solid line) and polyP CD spectra recorded at 90 and 25 °C (dashed line). The shared maxima at about 206 nm and minima at about 223 nm indicate that the structural differences that occur during heat-induced polyP film formation are comparable to those between HTTex1 N and T-fibrils.
