Fig. 1: Biochemical characterization and cryo-EM structure.
From: Structural basis for recognition and regulation of arenavirus polymerase L by Z protein
a Elution profile of the purified JUNV L protein on a Superose 6 Increase 10/300 GL size-exclusion column. SDS-PAGE was performed to assess the quality of L protein (repeated ≥ 3 times). b Elution profile of the purified JUNV L–Z complex on a Superose 6 Increase 10/300 GL size-exclusion column. SDS-PAGE was performed to assess the quality of L–Z complex (repeated ≥ 3 times). c A representative negative staining micrograph of JUNV L–Z complex (repeated ≥ 3 times). d A representative cryo-EM micrograph of JUNV L–Z complex (out of ~6400 micrographs). e The final cryo-EM density map of JUNV L–Z complex.
