Fig. 5: Schematic diagram showing features of JUNV L protein for RNA synthesis.
From: Structural basis for recognition and regulation of arenavirus polymerase L by Z protein
a Tunnels within the JUNV L structure for RNA synthesis. The template entrance and exit (black), the NTP entrance and product exit (blue) are shown. The catalytic site is indicated by a red star. b The product exit is blocked by Z protein. The protein structures are shown as cartoon and colored by domains, the catalytic site is indicated by a red star. The template entrance, NTP entrance, template exit and product exit are indicated by arrows. c Comparison of the 680-loop of MACV L protein and JUNV L–Z complex. Superimposition of the apo MACV L protein on the JUNV L–Z complex. The black dot box indicates magnified views of the 680 loops of the JUNV L protein (shown in light purple) or MACV L protein (shown in light pink), and displays the steric hindrance between MACV L and JUNV Z protein (shown in blue). d Sequence alignment of the region around the 680-loop. The conserved residues were indicated by red triangles. GTOV, Guanarito virus; WWAV, Whitewater Arroyo virus; PIRV, Pirital virus; SABV, Sabia virus; OLIV, Oliveros virus; LUJV, Lujo virus; MBLV, Mobala virus; MOPV, Mopeia virus; LCMV, lymphocytic choriomeningitis virus; IPPYV, Ippy virus.
