Fig. 1: Expressions of miR-455-5p and -3p in chondrocytes.

a Heatmap of miRNA microarray. The microarray data of differentially expressed miRNAs (Signal value: > 1.5-fold and < 0.6-fold change in MOI = 5, and > threefold and < 0.6-fold change in MOI = 20) whose expression changed by adenovirally expressed Sox9 are shown. LacZ-expressing chondrocytes were used as a negative control. b Relative expression of miRNAs in Sox9- or LacZ-expressing adenovirus-infected chondrocytes. The experiment was performed once with three biological replicates (n = 3). Data are represented as the mean ± SEM. ns not significant, *P < 0.05, **P < 0.01, ***P < 0.001. Two-tailed Student’s t test. c Relative expression of both strands of miRNAs in mouse primary chondrocytes. The experiment was performed independently twice with two biological replicates (n = 4). d Relative expression of miR-455s and miR-140 in OA (n = 11, biologically independent samples) or normal articular cartilage (N; n = 10, biologically independent samples). Data are represented as the mean ± SEM. Two-tailed Student’s t test. e Relative expression of pri-miR-455, Col27a1, mature miR-455s, Sox9, Col2a1 and Mmp13 in mouse primary chondrocytes with or without IL-1β stimuli (IL-1β or Ctrl). For miR-455s and Col27a1, the experiment was performed independently twice with two or three biological replicates (n = 5). For other cartilage-related genes, the experiment was performed once with three biological replicates (n = 3). Data are represented as the mean ± SEM. Two-tailed Student’s t test. Source data are provided as a Source Data file.