Fig. 2: miR-455 knockout mice show an OA-like phenotype. | Nature Communications

Fig. 2: miR-455 knockout mice show an OA-like phenotype.

From: Both microRNA-455-5p and -3p repress hypoxia-inducible factor-2α expression and coordinately regulate cartilage homeostasis

Fig. 2

a Genetic deletion in miR-455-deficient mice generated by CRISPR/Cas9. b Representative image of Safranin-O staining of wild-type (WT) and miR-455 knockout (KO) mice (2, 6-month-old). Staining was repeated at least three times with similar results. Scale bars show 200 μm. c The OARSI scores of 2 (n = 10, biologically independent samples) or 6-month-old (n = 12, biologically independent samples) wild-type (WT) and miR-455 knockout (KO) mice (2, 6-month-old). Data are represented as the mean ± SEM. Two-tailed Student’s t test. d Relative mRNA levels of cartilage markers and cartilage degeneration-related genes in knee cartilage of wild-type (WT) and miR-455 knockout (KO) mice (2, 6-month-old). Data are represented as the mean ± SEM, n = 5 biologically independent samples. ns: not significant, *P < 0.05, **P < 0.01. Two-tailed Student’s t test. Source data are provided as a Source Data file.

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