Fig. 5: Potential therapeutic effect of miR-455s in OA. | Nature Communications

Fig. 5: Potential therapeutic effect of miR-455s in OA.

From: Both microRNA-455-5p and -3p repress hypoxia-inducible factor-2α expression and coordinately regulate cartilage homeostasis

Fig. 5

a Schedule of treatment using transfection of miR-455s mimic in a surgically induced OA model. The mouse figure is a partial modification of the figure created by Sho Mokuda in the previous paper65. b Representative image of Safranin-O staining. Staining was repeated at least twice with similar results. Scale bars show 200 μm. c OARSI scores. Data are represented as the mean ± SEM, n = 12, biologically independent samples. One-tailed Dunnett’s test. Ctrl: control, 5p: miR-455-5p, 3p: miR-455-3p, 5p/3p: miR-455-5p and -3p. d Representative image of Hif-2α or MMP13 staining. Staining was repeated at least twice with similar results. Scale bars show 100 μm. e Percentages of Hif-2α and MMP13-positive cells. Data are represented as the mean ± SD, n = 5, biologically independent samples. Two-tailed Student’s t test. Ctrl: control, 5p/3p: miR-455-5p and -3p. Source data are provided as a Source Data file.

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