Fig. 4: TATA box motif leads to long durations of a permissive promoter state.
a–c Representative traces of single-nuclei transcriptional activities for the indicated genotypes. Gray boxes indicate the analyzed transcriptional windows, black curves represent the signal expressed as the number of polymerases, and red curves represent the reconstructed signal after the deconvolution procedure. d–f Heatmaps indicating the number of polymerases during nc14 for each genotype. g–i Representation of estimated bursting parameters for sna (g), snaTATAlight (h), and snaTATAmut (i) promoters (see also Supplementary Table 1 and Supplementary Table 2). j Duration and probability of the permissive ON state. Error bars represent the smallest and largest values in optimal and close to optimal solutions (see “Methods”). k Duration and probability of the OFF state. Error bars represent the smallest and largest values in optimal and close to optimal solutions (see “Methods”). l Estimated burst size calculated as kINI/kOFF75 using the optimal kinetic parameters (Supplementary Table 2). Statistics: snaE < sna, 216 nuclei, 3 movies; snaE < snaTATAlight, 353 nuclei, 6 movies; snaE < snaTATAmut, 21 nuclei, 3 movies. See Supplementary Movies 1–3 and Supplementary Table 2.
